Figure 1
From: Transcriptional landscape of bone marrow-derived very small embryonic-like stem cells during hypoxia
Isolation of VSELs from peripheral blood (Panel A) and bone marrow (Panel B) using FACS. Forward scatter (FSC) and side scatter (SSC) distribution of beads with a range of diameters (1-15 μm, not shown) identified region 1 (R1) and was used to select populations of peripheral blood (Panel A) or bone marrow mononuclear cells (Panel B) ranging in size between 2-10 μm (R1). The small, low complexity cells in R1 were further separated using expression of Sca-1 and Lineage markers (R2: Sca-1+ Lin-), followed by sorting using CD45 marker expression into two distinct populations (R3, R4): hematopoietic stem cells (HCS, Sca-1+ Lin- CD45+), and very small embryonic-like stem cells (VSELs, Sca-1+ Lin- CD45).