Figure 1

Prevalence of pulmonary MCs in IPAH patients. Lung tissues from donors and IPAH patients were stained with toluidine blue (TB). The arrow indicates the positive signal (purple/violet stain) for the TB-stained MCs. (A) Representative photomicrographs of lung sections from donor (a) and patients (b) are shown. (B) Total and (C) perivascular MC count of different vessel size are given. (D) Perivascular MCs were further analyzed to identify granulated (a) and degranulated (b) MCs, and an index of granulation (IOG) was determined. (E) IOG (in %) is shown. Each bar represents Mean ± SEM (n = 10-15). *p < 0.05 versus donor/corresponding vessels of donor. Scale = 20 μm.