Table 1 Primers used for RT-PCR
Name | Primer sequence | Melting temperature (°C) | MgCl 2 (mM) | Product size (base pairs) |
|---|---|---|---|---|
β-actin | F:GGAAGGCTGGAAGAGTGC R:CTACAATGAGCTGCGTGTGG | 56 | 2 | 528 |
hCAP18/LL-37 | F:GGCTCCTTTGACATCAGTT R:CTGTCCTGGGTACAAGATTCC | 55 | 2.5 | 150 |
hBD-2 | F:ATCAGCCATGAGGGTCTTG R:GCAGCATTTTGTTCCAGG | 62 | 2 | 190 |
hBD-3 | F:AGCCTAGCAGCTATGAGGATC R:CTTCGGCAGCATTTTGCGCCA | 62 | 2 | 206 |
MUC5AC | F:ATTTTTTCCCCACTCCTGATG R:AAGACAACCCACTCCCAACC | 58 | 2 | 146 |
MUC2 | F:GACGCACTGTATCATCAAACG R:AGGATGGTCGTGTTGATGCG | 58 | 2 | 409 |
CBE-1 | F:GGTCAGAGCTTGAGGTAGCATGTG R:GAGTTGTAACAGCACACTGCATTC | 60 | 3 | 395 |
Tektin | F:AGCTGGCTGATCATCTGGCCAAG R:GGATTTCCTCATCTGCATACACAG | 60 | 2 | 358 |