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Figure 1 | Respiratory Research

Figure 1

From: IL-4 and IL-13 exposure during mucociliary differentiation of bronchial epithelial cells increases antimicrobial activity and expression of antimicrobial peptides

Figure 1

Schematic representation of the air-liquid interface (ALI) culture model. Primary bronchial epithelial cells were seeded onto transwell filters and allowed to grow to confluence for 4-7 days. Cells were exposed to air at the apical side (air-liquid interface; ALI) and stimulated for 14 days at the basal side with medium (containing high concentrations of retinoic acid to induce mucociliary differentiation). To investigate effects of IL-4 or IL-13 on differentiating cells, IL-4 or IL-13 was added to the basal medium when cells were first exposed to air; medium with or without IL-4 or IL-13 was refreshed three times a week.

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Respiratory Research

ISSN: 1465-993X

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