Figure 3
From: Pulmonary arterial dysfunction in insulin resistant obese Zucker rats
Endothelial function and eNOS protein expression. (A) Concentration-response curve to acetylcholine in endothelium intact conductance PA rings precontracted with phenylephrine 10-7M (left) and resistance PA rings precontracted with phenyleprine to reach a 75% of KCl contraction with or without L-NAME 10-4 M (right) from lean and obese Zucker rats (n = 4-6). (B) Concentration-response curve to sodium nitroprusside in conductance PA rings contracted by 5-HT (10-4M) in the presence of L-NAME (10-4M, n = 5). (C) eNOS mRNA levels in resistance PA analyzed by RT-PCR and normalized by β-actin expression (n = 5) and (D) eNOS protein expression from whole lung homogenated analyzed by Western blot and normalized by β-actin expression (n = 8). Results indicate mean ± s.e.m.