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Table 2 List of the antibodies, concentrations and antigen-retrieval methods used in the study.

From: Tenascin-C and alpha-smooth muscle actin positive cells are increased in the large airways in patients with COPD

Antibody

Source

Concentration

Antigen retrieval

α-SMA

Dako

1:1000

MW 19 min in tris-EDTA‡

Desmin

Dako

1:300

MW 19 min in tris-EDTA

Tn-C

Biohit

1:1000

MW 30 min in tris-EDTA

Vimentin

Dako

1:1500

MW 14 min in citrate†

  1. MW = microwave heat treatment; ‡ = tris/EDTA buffer, pH 9.0; † = citrate buffer, pH 6.0
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Respiratory Research

ISSN: 1465-993X

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