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Figure 4 | Respiratory Research

Figure 4

From: Pro-inflammatory endothelial cell dysfunction is associated with intersectin-1s down-regulation

Figure 4

NeutrAvidin Alexa Fluor 594 staining demonstrates inhibition of caveolae internalization by 1 μg/ml LPS exposure. NeutrAvidin Alexa Fluor 594 staining of control ECs subjected to biotinylation of cell surface proteins followed by internalization assay, indicates a strong punctate pattern throughout the cytosol (c), with some accumulation in the perinuclear area. An enlarged ECs (boxed area in c) subjected to the internalization assay is shown in c1. ECs exposed to LPS and subjected to the internalization assay followed by neutrAvidin Alexa Fluor 594 staining (b, b1) show large fluorescent puncta within the intracellular space with no perinuclear accumulation. Bars: a, a1, b, b1 - 20 μm.

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