Figure 3

Effects of 1 μg/ml LPS exposure causes significant inhibition of caveolae internalization. A. Control and LPS-treated cells were subjected to biotinylation of cell surface proteins as described under Materials and Methods, followed by internalization of biotinylated proteins for 30 min, at 37°C. The number of biotin molecules present in lysates of control and LPS-treated cells was quantified by ELISA using streptavidin-HRP Ab, in 3 different experiments. The results were normalized per milligram total protein, per 30 min. Ordinate, amounts of biotin molecules/well detected per well; abscissa, μg total protein/well as result of serial dilution of control and LPS-treated ECs lysates. B. Degree of inhibition of caveolae-mediated uptake in LPS-treated ECs by reference to controls. Bars, ±SE.