Figure 4

Double immunofluorescence staining for detection of IL-17A and IL-17F expression in CD4⁺ and CD8⁺ T cells in airways of COPD patients. (A) Double immunofluorescence staining was performed. Scale bar = 5 μm. (B) Percentage of CD4⁺ and CD8⁺ T cells that express IL-17A and IL-17F. Results are expressed as means ± SEM. (C) Percentage of CD4⁺ and CD8⁺ T cells that express IL-17A and IL-17F in total IL-17A⁺ and IL-17F⁺ cells. Results are expressed as means ± SEM. *P < 0.05. N = 3 COPD patients. (D) IL-17A and IL-17F mRNA expression in CD8⁺ T cells in airways of COPD patients. Immunohistochemistry determined CD8⁺ T cells were selected by LCM, and then RT-PCR was performed to detect the mRNA expression of IL-17A and IL-17F. One representative result from 3 subjects is shown.