Figure 4

Anti-OVA IgG1 inhibited the cell proliferation of BMDCs in vitro. BMDCs (2 × 10⁴ cells per well) were purified from WT mice and pulsed with OVA peptide (20 μM) or anti-OVA IgG1 (0.1 mg/ml) containing the same amount of OVA. Negative control means no addition of OVA peptide in culture medium. Cells were washed and used to stimulate CD4⁺ T-cells (2 × 10⁵ cells per well). The proliferation of T cells after DCs treatment with anti-OVA IgG1 was quantified in duplicate by measuring the optical density using the WST-8 cell-counting kit after 48 h. Data show means ± SEM. *p < 0.05.