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Figure 5 | Respiratory Research

Figure 5

From: Adrenomedullin in inflammatory process associated with experimental pulmonary fibrosis

Figure 5

Effects of adrenomedullin (AM) on bleomycin (BLM)-induced nitrotyrosine and PAR formation. Immunohistochemical analysis of lung sections obtained from mice treated with BLM revealed positive staining for nitrotyrosine (B). In BLM mice treated with AM, positive staining for nitrotyrosine was significantly reduced (C). Moreover, immunohistochemical analysis of lung sections obtained from mice treated with BLM revealed a positive staining for PAR (F). In contrast, positive staining for PAR was significantly reduced in the lungs of BLM mice treated with AM (G). No positive staining for nitrotyrosine (A) and PAR (E) was observed in lung tissues obtained from sham-operated mice. Densitometry analysis (D and H) of immunohistochemistry photographs (n = 5 photos from each sample collected from all mice in each experimental group) for nitrotyrosine and PAR was assessed. The assay was carried out by using AxioVision on a personal computer. The figure is representative of at least three experiments performed on different experimental days. Data are expressed as % of total tissue area and are mean ± standard deviation from n = 10 mice for each group. *P < 0.01 vs sham, °P < 0.01 vs bleomycin+ vehicle.

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