Figure 7
From: Some ABCA3 mutations elevate ER stress and initiate apoptosis of lung epithelial cells
Apoptotic signaling in cells with L101P and R280C mutations is activated by ER stress. (A) Immunoblotting on whole cell lysates from A549 cells transfected with pEYFP-N1/ABCA3 and densitometric analyses of (B) pro-caspase 4 and (C) cleaved caspase 4 demonstrated increased caspase 4 cleavage in cells expressing L101P and R280C mutations, as well as after TNFĪ± treatment of A549 (25 ng/ml, 16 h) in comparison to WT and untreated A549. No significant changes in the pro-caspase 4 level in transfected cells with WT, R43L, R280C and L101P mutations were detected but pro-caspase 4 was slightly increased in transfected cells compared to A549 (B). * - unknown band. Six independent experiments were used for densitometric evaluation; * p < 0.05, ** p < 0.01.