Figure 2
From: Some ABCA3 mutations elevate ER stress and initiate apoptosis of lung epithelial cells
Processing of the WT and mutant R43L, R280C and L101P ABCA3. (A) Immunodetection with anti-GFP antibody showed two ABCA3 protein bands (180 kDa and 220 kDa) in whole cell lysates of A549 cells expressing WT and R43L and R280C mutations, and only one protein band in the cells expressing L101P mutation. (B) Deglycosylation assay with PNGaseF and EndoH on the membrane fractions from A549 cells transfected with pEYFP-N1/ABCA3 plasmids and subsequent ABCA3-YFP immunodetection with anti-GFP antibody showed presence of high-mannose and complex oligosaccharides in WT, R43L and R280C proteins, as well as only high-mannose and no complex oligosaccharides in the L101P mutant resulting from the L101P ER retention.