Role of the gap junctions in the contractile response to agonists in pulmonary artery from two rat models of pulmonary hypertension

Table 1 Sequences of the primer pairs (S: sense; AS: antisense) for housekeeping genes (GAPDH, HPRT, PLRPO and YWHAZ) and genes of interest (Cx 37, Cx 40 and Cx 43) are shown as well as GenBank accession number, product length, product Tm and concentrations

Gene	Sequence	GenBank accession number	Product length (pb)	Product Tm (°C)	Concentration (nM)
GAPDH	S: ATTCTACCCACGGCAAGTT	NM_017008	153	89.4	200
	AS: CGCCAGTAGACTCCACGACATA
HPRT	S: TGTTGGATATGCCCTTGACTA	NM_012583	178	85.6	100
	AS: AGATGGCCACAGGACTAGAAC
PLRPO	S: AGGTGGGAGCCAGCGAAGC	NM_022402	208	91.7	100
	AS: GCAACAGTCGGGTAGCCAATC
YWHAZ	S: AGCCGAGCTGTCTAACGAG	NM_013011	291	88.4	100
	AS: GCCAAGTAGCGGTAGTAGTCA
Cx 37	S: GGTGGCAGAGGACGGTCGTCT	NM_021654	133	85.3	200
	AS: CCATGGTCCAGCCGTAGAGA
Cx 40	S: GGAAAGAGGTGAACGGGAAG	NM_01280	197	91.3	200
	AS: GGGCCTCGAGACATAACAGTT
Cx 43	S:TCTGCCTTTCGCTGTAACACT	NM_012567	117	87.5	200
	AS: GGGCACAGACACGAATATGAT

ISSN: 1465-993X