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Figure 2 | Respiratory Research

Figure 2

From: Role of the gap junctions in the contractile response to agonists in pulmonary artery from two rat models of pulmonary hypertension

Figure 2

Localization of Cx 37, 40 and 43 in the pulmonary arterial wall. Immunofluorescent labelling experiments shows the presence of Cx (red) 37 (top row), 40 (middle row) and 43 (bottom row) in transverse sections of pulmonary arteries from normoxic (normoxia, left column), chronic hypoxic (chronic hypoxia, middle column) and monocrotaline-injected rats (monocrotaline, right column). Green shows the autofluorescent signal of the vessels and blue shows the nuclei. SMC means smooth muscle cells and EC means endothelial cells. 7-8 normoxic rats have been used and the number of IPA3 sections, used for the labelling of Cx 37, 40 and 43, was 21, 22 and 14 respectively. 4-5 monocrotaline-injected rats have been used and the number of IPA3 sections, used for the labelling of Cx 37, 40 and 43, was 11, 17 and 14 respectively. Finally, we have used 3 chronic hypoxic rats and the number of IPA3 sections used for the labelling of the Cx 37, 40 and 43, was 4, 5 and 7 respectively. Scale bars are 15 μm.

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