Figure 5

Effect of albuterol on AHR in non-allergic mice. Respiratory mechanics time course following methacholine challenge. Animals were treated with albuterol twice daily for seven days with the last administration 18 hours before experiment. Parameters from fitting the constant phase model to input impedance data; R _n is Newtonian resistance of the conducting airways; H is lung elastance and G is tissue resistance. Left column: AHR assessment in naïve C57Bl/6 mice, treated with (RS)-, (R)-, (S)- albuterol or control PBS (n = 8, 6, 7 and 8). Middle column: AHR assessment in naïve A/J mice. Animals were treated with (RS)-, (R)-, (S)- albuterol or control PBS (n = 8, 5, 7 and 7). Right column: AHR assessment in Balb/C mice pretreated oropharyngeally with Poly-L-lysine (PLL) (50 μg in 50 μl PBS). PLL was administered once daily for 4 consecutive days before the assessment of respiratory mechanics with methacholine. Animals were treated with (RS)-, (R)-, (S)- albuterol or control PBS (n = 6, 6, 7 and 8).