Figure 1

Timelines of the experiments. A) To measure the immediate allergic airways response (IAR) Balb/C mice were immunized with OVA + Alum i.p. on days 0 and 14. On days 21-26, animals were exposed for 30 minutes to 1% aerosolized OVA; controls received PBS aerosol. Four different groups of mice were treated with nebulized albuterol, (R)- (2.5 mg/ml), (S)- (2.5 mg/ml), (RS)- (5 mg/ml) or control PBS, twice daily for 20 minutes in the morning and in the afternoon on days 21-27. Lung mechanics was measured on day 28 following inhalation of 5% OVA aerosol. B) To measure the effect of albuterol on allergen induced AHR, Balb/C mice were immunized with OVA + Alum i.p. on days 0 and 14. On days 21-23 animals were exposed for 30 minutes to 1% aerosolized OVA; controls received PBS aerosol. The mice were treated with nebulized albuterol, (R)- (2.5 mg/ml), (S)- (2.5 mg/ml), (RS)- (5 mg/ml) or control PBS, twice daily for 20 minutes in the morning and in the afternoon on days 18-24. AHR was assessed by measuring Z _rs at increasing doses of inhaled methacholine (MCh).