Figure 2

Plexiform-like lesions occur in female Mts1+ mice. A: Images of lung sections taken from female and male Mts1+ mice and stained with α-smooth muscle actin (x200 magnification). A cluster of plexiform-like lesions can be seen in the female Mts1+ mouse, whereas pulmonary arteries of a similar size in the male mouse show a much smaller smooth muscle cell layer and an intact lumen. Scale bars represent 100 μm. B-E: Representative images (x400 magnification) of plexiform-like lesions observed in female Mts1+ mice. Plexiform like lesions show extensive α-smooth muscle actin staining in the neointimal layer (B). Staining with von-Willebrand factor shows endothelial cells lining the obstructed lumen of the lesion (C). Mts1 (D) and RAGE (E) staining are evident in the endothelial and adventitial layers of the plexiform-like lesions Scale bars represent 50 μm.