Table 1 List of primers used for measuring gene expression by real time PCR
From: Impaired neonatal macrophage phagocytosis is not explained by overproduction of prostaglandin E2
Gene name | Forward Primer | Reverse Primer |
|---|---|---|
β-actin | CCTAAGGCCAACCGTGAAAA | AGGGACAACACAGCCTGGAT |
COX-1 | GACCTGCCCCTATGTCTCCTT | TCACGAAGACAGACCCGTCAT |
COX-2 | GGATCCCCAAGGCACAAATA | CCTCGCTTCTGATCTGTCTTGA |
5-LO | GCTGCACAGAGTTGCCTAAGAA | TCTAAACTGAGCTGCCGCTCTA |
FLAP | CCTTCGCTGGGCTGATGT | TCTCCCAGATAGCCGACAAAGT |
mPGEs-1 | AGGGTGCCATGTTTTCAGAG | CAGTCTTTGGAGGAGCCAAG |
EP1 | AGCAGATGTGAGGGCAGAGT | GCCTCATCCACTAGGCTCAG |
EP2 | CCTGGCCATTATGACCATCAC | TCGGGAAGAGGTTTCATCCA |
EP3 | GTCTAGGCTTGCTGGCTCTG | TGCGTCTTGCATTGCTCTAC |
EP4 | ACGCGGGCTTCAGTTCCT | CGCACACCAGCACATTGC |
BLT1 | AGGATGCAGAAACGCTCTGT | CGGTCCAGGCTCATGATAGT |
cys-LT 1 | GCCTCACCACCTATGCCTTA | CCTGTGGAGGGCTCAAAACAT |
Fcγ receptor | CCTGCAGAGTCCTGGCTTAC | CTGATGACTGGGGACCAAAT |