Figure 5

Modulation of eicosanoids did not alter phagocytosis of young AMs. AMs were harvested from adult ((~10 weeks old) and young (day 7 and 14) rats and allowed to adhere overnight. A and B. AMs were either untreated or treated with 200 μM aspirin (A) or 10 nM LTB₄ (B) for 20 min before the addition of an opsonized target. The amount of ingestion of the target was quantified after 90 mins. For phagocytosis assay, data are presented as the percent of increase above baseline phagocytosis for each age group and shown are the mean ± SEM from 3 independent experiments preformed in triplicate. C. RNA was extracted from the cells, converted into cDNA and the relative expression was determined using semi-quantitative real-time PCR. For each sample, the expression of Fcγ receptor was normalized to β-actin and the average of the adult AMs was set to 1. For real time PCR, data represents the mean ± SEM of pooled samples from 3 independent experiments (**p < 0.01 and ***p < 0.001 when untreated group).