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Figure 6 | Respiratory Research

Figure 6

From: Cigarette smoke increases TLR4 and TLR9 expression and induces cytokine production from CD8+T cells in chronic obstructive pulmonary disease

Figure 6

TLR4 and TLR9 protein expression on CD8+ T cells is upregulated by CSC. CD8+ T cells isolated from the peripheral blood of COPD patients (n = 4-5) and treated with CSC (10 μg/ml or 50 μg/ml) or DMSO for 24 hours. A representative western blot nitrocellulose membrane expressing TLR4, TLR9 and GAPDH (A). Western blot analysis revealed an increase in TLR4 (B) and TLR9 (C) protein expression with a CSC treatment of 50 μg/ml. Individual samples were normalized to GAPDH and then expressed as a relative ratio of treated compared to DMSO sample. Data are represented as mean ± SEM. * indicates p-values < 0.05.

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