Figure 2
Proinflammatory cytokine treatment exacerbated pulmonary dysfunction in EV71-infected, but not HSV-1-infected mice. Seven day-old ICR mice (n = 12) were intracranially inoculated with EV71 (4 × 105 PFU/mouse) or HSV-1/KOS (2 × 102 PFU/mouse) followed by an intraperitoneal injection of IL-6, IL-13, and IFN-γ at days 3 and 4 post inoculation (arrows). The body weights (A) and pulmonary functions of mice were monitored daily (B). Ctrl: culture medium; BSA: bovine serum albumin; Cyt: IL-6, IL-13, and IFN-γ treatment; PIF: peak of inspiratory flow; PEF: peak of expiratory flow; MV: minute volume; TV: tidal volume. Data represent means ± S.E.M. *, P < 0.05 and **, P < 0.0001 as compared to BSA-treatment control. #, P < 0.01 and ##, P < 0.001 as compared to EV71-infected mice.