Figure 3

AR deficiency prevents accumulation of eosinophils in BAL of RWE-challenged mice. The mice were sensitized and challenged with PBS or RWE and 72 h later lungs were lavaged and differential cell counts were determined as described in methods. The total cells (A) and eosinophils (B) per ml BAL fluid are shown as bar diagram. The bars represent mean ± SD (n = 4-6). **p <0.001. (C) The photomicrograph showing a representative field of H&E stained preparations from each group of mice (Magnification 200×). Arrowheads point to eosinophils in BAL cytospin preparation (n = 4-6). RWE, ragweed pollen extract; WT, wild-type; AR^-/-, aldose reductase null mice.