Figure 5
From: Epilysin (matrix metalloproteinase-28) contributes to airway epithelial cell survival
MMP28 expression in the lung decreases in influenza infection and does not contribute to viral replication or clearance. C57BL/6 wild-type and Mmp28-/- mice were infected with 100 PFU of mouse-adapted H1N1 influenza (A/PR/8/34), and the lungs were harvested at serial time-points for RNA isolation. (A) Expression of MMPs -3,-7,-12, and -28 was assessed using real-time PCR. Only MMP28, which is constitutively expressed in the naïve lung, decreased during infection. (n = 4 mice/time-point). P value <0.05 for all MMPs when compared to their respective baseline expression at D0, except for MMP7 at D4. (B) Viral titers as assessed by RT-PCR from lung homogenates did not differ between wild-type and Mmp28-/- mice (n = 4-5 mice of each genotype/time-point).