Figure 3

Expression of wild-type MMP-28 protects against staurosporine-induced apoptosis in A549 and BEAS-2B cells. A549 (A, B) or BEAS-2B (C) cells expressing wild-type MMP-28 (closed circle) or mutant MMP-28 (open circle) were plated on a 96-well plate with increasing doses of staurosporine in serum-free medium (0 - 2 μM). After 16 h incubation with staurosporine or serum-free medium, the level of caspase 3/7 activity was determined by measuring the fluorescence of a cleaved caspase 3/7 substrate (A,C). In separate experiments, the cell viability was assessed by Alamar Blue staining (B).