Figure 1

Representative images demonstrating expression of recombinant, V5-tagged wild-type and mutant MMP28 in A549 cells. A. Immunofluorescence (IF) using FITC-conjugated anti-V5 antibody. Bottom right image demonstrates localization of MMP28 to the plasma membrane. B. Western blot (WB) of supernatants (S) and cell lysates (L) from representative mutant and wild-type clones. Membranes were stained with anti-V5 HRP. The larger band (58 kDa) represents the pro-form, which is more abundant in the cell lysate, and the smaller band (48 kDa) represents the secreted active form, which is more abundant in the supernatant. A549 cells had no staining by IF or WB using the V5 antibodies.