Figure 1
Representative low magnification overview of a lung section from a patient with CF, stained with Masson's trichrome staining. Panel A-E show representative micrographs in different anatomical compartments of the lung from a patient with CF: (A) small airway, (B) pulmonary vessel, (C) normal alveolar parenchyma, (D) region of inflammation in alveolar parenchyma and (E) region of fibrosis in alveolar parenchyma. Consecutive sections were used for immunohistochemical double staining of tryptase-positive mast cells (MCT: permanent red) and chymase-positive mast cells (MCTC: DAB- brown) shown to the right in each panel. F represents a close-up image (600×) of neighbouring MCTC and MCT cells. Scale bars: Overview = 2000 μm, A-E = 100 μm. lu: airway lumen; ep: small airway epithelium; v: pulmonary vessel; and alv: alveolar parenchyma.