Figure 2
From: Pleiotropic effects of statins in distal human pulmonary artery smooth muscle cells
Effects of modulating the mevalonate pathway and combination therapy on DNA synthesis in human PASMCs. (A-B) Effect of simvastatin and reversal by the addition of mevalonate (MVA, 100 μM) or geranylgeranylpyrophosphate (GGPP, 10 μM), but not by squalene (Squal, 100 μM) or farnesylpyrophosphate (FPP, 10 μM). (C) Inhibitory effect of exoenzyme C3 (C3) and Rho-kinase inhibitor Y-27632. (D) Inhibitory effect of iloprost in combination with sildenafil or simvastatin and co-treatment with all three agents. Data are mean ± SEM of replicate values (n = 6), representative of independent experiments, and were obtained using IPAH (D) and non-IPAH (A-C) cells.