Figure 4From: Krüppel-like Factor 5 contributes to pulmonary artery smooth muscle proliferation and resistance to apoptosis in human pulmonary arterial hypertensionKLF5 inhibition promotes apoptosis. A) Mitochondrial membrane potential was measured by TMRM in confocal microscopy. PAH- PASMC are more hyperpolarized than control (more red) and KLF5 inhibition (siRNA) depolarized PAH-PASMC. B&C) Apoptosis was also evaluated by TUNEL and Annexin V staining in PASMC. PAH-PASMC are more resistant to starvation induced apoptosis (0, 1%FBS) than control PASMC. KLF5 inhibition in PAH-PASMC promotes apoptosis. D) Survivin expression (measured by Western blot) is increased in PAH-PASMC compared to control cells and KLF5 inhibition (siRNA) decreased survivin expression. Western blot were normalized to SM-actin (*, P < 0.05; ***, P < 0.001).Back to article page