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Figure 1 | Respiratory Research

Figure 1

From: Network analysis of quantitative proteomics on asthmatic bronchi: effects of inhaled glucocorticoid treatment

Figure 1

Schematic overview of the experimental workflow. The workflow used in the proteomics analysis of human endobronchial biopsies. Proteins were extracted and digested, before the peptides were labelled with iTRAQ® Reagents in a four-plex set. Each four-plex, containing one reference pool and three samples, were fractionated using ion exchange chromatography. Each fraction was then subjected to nano LC-MS/MS. The resulting spectra were searched for identification and quantification. The identified and quantified proteins were then analysed using Ingenuity Pathways Analysis.

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