Protease-activated receptor 2 activation of myeloid dendritic cells regulates allergic airway inflammation

Respiratory Research

Table 2 Differential cell count in BAL fluid from wild type and PAR2-deficient mice adoptively transferred with mDCs pulsed with PBS or GC frass.

	Mac	Epi	Eos	Neut	Lymph
BALB/c DC PBS	2.2 ± 0.8	13.0 ± 3.5	0	0.1 ± 0.06	0.3 ± 0.2
PAR2-/- DC PBS	3.5 ± 1.3	14.3 ± 2.8	0	0.1 ± 0.03	0.2 ± 0.05
BALB/c DC frass	5.2 ± 2.1	9.8 ± 2.1	1.6 ± 0.4	9.5 ± 2.1 *	3.4 ± 0.5
PAR2-/- DC frass	3.8 ± 1.4	12.8 ± 3.5	0.7 ± 0.2 †	7.5 ± 1.6 *	3.0 ± 0.4

Bone marrow was isolated from BALB/c and PAR-2-/- mice and was cultured in the presence of GM-CSF for 6 days. Cells were pulsed with PBS or GC frass (1 μg/ml) for an additional 24 hours. Wild type mice were sensitized to wild type and PAR-2-/- BMDC (1 × 10⁶ cells) pulsed with PBS or GC frass on Day 0. Fourteen days later, mice were challenged with an exposure to PBS or GC frass (40 μg/40 μl). On day 17, BAL fluid was harvested and differential cell counts performed. These data represent 8 mice per group and are expressed as mean ± SEM of cell number × 10³. Cell counts were statistically significant between GC frass-pulsed BMDC and PBS-pulsed BMDC (*p < 0.05) and between GC frass-pulsed wild type BMDC and GC frass-pulsed PAR-2-/- BMDC (†p = 0.015).

ISSN: 1465-993X