Figure 6
Adoptive transfer of GC frass-pulsed PAR-2-deficient mDCs had attenuated airway responses compared to GC frass-pulsed wild type mDCs. Naïve mice were sensitized with PBS- or GC frass-pulsed BMDC from wild type or PAR-2-/- mice on day 0. Mice were challenged with PBS (40 μl) or GC frass (40 μg/40 μl) on day 14. On day 17, mice were anesthetized and acetycholine was injected after establishment of a stable airway pressure. In all cases, means ± SEM are reported (n = 4-6 mice/group) and statistical analysis was performed by ANOVA. A. AHR was measured as airway pressure time index (APTI) in cm-H2O × sec -1 (* p < 0.001; **p = 0.003). Lungs from the mice were excised; cells dissociated and maintained in a single suspension culture for 3 days in the presence of GC frass (1 μg/ml). Cell supernatants were collected, clarified and analyzed by ELISA (in all cases *p < 0.001, *p < 0.05). B. IL-13 levels. C. IL-5 levels. D. IL-17A levels. E. IFNγ levels.