Figure 3

Frass treatment increased expression of PAR-2 on pulmonary myeloid dendritic cell. Lungs from PBS or GC frass-treated mice were harvested 20 h post exposure and stained for flow cytometric analysis of PAR-2 expression. A. Representative histogram showing PAR-2 expression on gated pulmonary mDCs from PBS-treated (solid line) or GC frass-treated (dashed line) mice. Solid grey histogram depicts staining with isotype control mAb. B. Average PAR-2 MFI on gated pulmonary mDCs. Data are expressed as Mean ± SEM (n = 6 mice per group) and statistical significance determined by ANOVA (*p < 0.001). C +D. AF405-labeled GC frass was instilled in the airways of wild type or PAR-2-/- mice and 20 h post exposure lungs were isolated and stained for flow cytometry. C. Percent of AF405-positive mDC in the lung (*p < 0.05). D. Mean fluorescence intensity (MFI) of AF405 in mDCs (*p < 0.05).