Figure 2
GC frass protease is an adjuvant for OVA-induced airway responses. Wild type mice were sensitized and challenged to LPS-free OVA, OVA plus enriched GC frass protease (OVA+protease) or OVA plus LPS (0.1 μg LPS; OVA+LPS) on Days 0, 7, and 14. On day 17, mice were anesthetized and acetycholine was injected after establishment of a stable airway pressure. In all cases the data are expressed as mean ± SEM (n = 4-6 mice per group) and statistical significant was determined by ANOVA. A. AHR was measured as airway pressure time index (APTI) in cm H2O × sec-1 (*p < 0.001 **p = 0.015). B. Serum IgE levels were measured by ELISA (*p < 0.05). Lungs from mice were excised, cells dissociated and maintained in a single suspension culture for 3 days unstimulated or in the presence of ConA (10 μg/ml). Cell supernatants were collected, clarified and analyzed by ELISA (in all cases, *p < 0.001 and ** p < 0.05). C. IL-5 levels. D. IL-13 levels. E. IL-17A levels. F. IFNγ levels.