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Figure 2 | Respiratory Research

Figure 2

From: Overexpression of sICAM-1 in the Alveolar Epithelial Space Results in an Exaggerated Inflammatory Response and Early Death in Gram Negative Pneumonia

Figure 2

Characterization of SPC-sICAM-1 transgenic mice. Transgene-specific primers demonstrated lung-specific expression of the SPC-sICAM-1 transgene in the lungs, with no expression detected in wild-type mice (a). Protein expression in SPC-sICAM-1 in BALF was increased (~2 log fold) over wild type mice (b, *** P < 0.05 by t-test), but did not significantly affect total BALF protein or serum sICAM-1 expression (c, d). Western analysis of BALF demonstrates a larger protein (~100 kDA, black arrowhead) in SPC-sICAM-1 BALF not present in wild type BALF. The lighter bands (white arrowhead) represent endogenous processing of sICAM-1. Whole lung mince from a wild type mouse is included for comparison. Two representative mice are shown for SPC-sICAM-1 (F1 generation) and wild type mice (e).

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