Figure 4

Immunohistochemical/immunofluorescent findings in lungs from PH models and controls. A: distribution of OX62⁺ cells in lungs of a control; DCs are detected within BALT. B: OX62-staining in MCT-rats; DCs are infiltrating the adventitia and are present in BALT. C: PA from a MCT/PE-animal; perivascular OX62+ cells are infiltrating adventitia and media of a remodeled vessel. D: double-labeling (anti-CD68/anti-actin) in a PA of a MCT/PE-animal: medial monocytic infiltration. E: double-labeling (anti-OX62/anti-actin antibodies in a PA (MCT/PE); DCs infiltrate the media. F: double-labeling (anti-CD68/anti-vimentine) in a PA (MCT/PE); cells within the media show co-localization of markers (yellow cells). G: anti-NOX-4 staining in a MCT/PE-animal. Interstitial NOX-4+ cells are highlighted. H: NOX-4+ cells next to a small arteriole (MCT/PE-animal). I: anti-NOX-4 staining in a control. Few cells display positivity. J: anti-Mn-SOD staining in a MCT/PE-animal: cells in the range of a remodeled PA are highlighted. K: A remodeled arteriole displays positivity for some perivascular cells (MCT/PE-animal). L: Control animal showing virtual negativity for Mn-SOD+ cells. M: anti-Tryptase staining reveals positive cells (same cytomorphology and distribution) next to a small diseased PA (MCT/PE-animal). N: Interstitial tryptase+ cells in the range of an arteriole (MCT/PE-animal). O: Immunofluorescent double-staining: merge-photo of a PA in a MCT/PE-animal. NOX-4 = red, CXCR4 = green and Dapi = blue: note NOX-4+/CXCR4+ cells. P: Smaller remodeled arteriole displaying cells of the same NOX-4/CXCR4+ phenotype in the perivascular space. Q: anti-SDF-1 staining (MCT/PE-animal). Endothelial cells of a diseased PA and a smaller vessel, inflammatory perivascular cells and bronchial epithelial cells are positive.