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Figure 2 | Respiratory Research

Figure 2

From: Simvastatin inhibits TGFβ1-induced fibronectin in human airway fibroblasts

Figure 2

Involvement of isoprenoid intermediates of the mevalonate cascade in the simvastatin-induced suppression of TGFβ1-induced fibronectin expression. (A) Representative western blot showing fibronectin protein abundance in whole cell lysates obtained from primary human airway fibroblast cultures stimulated with TGFβ1 (2.5 ng/ml) in the presence or absence of simvastatin (10 μM) and co-incubated with GGPP (30 μM), FPP (30 μM) or mevalonate (1 mM) for 48 h. (B) Data from densitometry of samples from experiments shown in panel A. Data represent means ± s.e. mean of 3 independent experiments, using 3 different cell lines. Legend: 0 h and t = 0: initial basal (unstimulated) cultures; 48 h and t = 48 h: treatment time-matched, basal (unstimulated) cultures; C: cells treated with TGFβ1 alone for 48 hours; Simv: simvastatin; Mev: mevalonate. ##P < 0.01 compared to C(ontrol); *P < 0.05 compared to simvastatin.

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