Skip to main content
Figure 4 | Respiratory Research

Figure 4

From: Endothelial-like cells in chronic thromboembolic pulmonary hypertension: crosstalk with myofibroblast-like cells

Figure 4

Human pulmonary microvascular ECs (HPMVECs) co-cultured with pulmonary arterial fibroblast-like cells (PAFLCs) or myofibroblast-like cells (MFLCs). The phenotypical alteration of HPMVECs was assessed microscopically and by immunofluorescence staining after a 14 day incubation period. A) and C), HPMVECs co-cultured with PAFLCs; B) and D), HPMVECs co-cultured with MFLCs; A) and B), Microscopic findings; the magnification was 100×. Scale bar = 100 μm; C) and D), Immunofluorescence staining for anti-Factor VIII (green) and anti-α-SMA (red). The blue staining was DAPI. The magnification was 400×. Scale bar = 25 μm. D), Some cells coexpressed both anti-Factor VIII and anti-α-SMA (see inset); HPMVECs = human pulmonary microvascular endothelial cells; MFLCs = myofibroblast-like cells; PAFLCs = fibroblast-like cells from control pulmonary arteries. E) Positive cells for anti-von Willebrand factor and anti-α-SM-actin were counted in 3 different fields at a magnification of × 200 in a fluorescence microscope. *P < 0.05 VS. PAFLCs, n ≥ 3.

Back to article page