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Figure 2 | Respiratory Research

Figure 2

From: Endothelial-like cells in chronic thromboembolic pulmonary hypertension: crosstalk with myofibroblast-like cells

Figure 2

ELCs from endarterectomized tissue. A-F), ELCs were assessed by immunofluorescence staining for anti-Factor VIII (green) and anti-α-SMA (red) to confirm the phenotypes of the cells. A), B) and C), ELCs before sorting; D), E) and F), ELCs after sorting; A) and D), the magnification was 100×. Scale bar = 100 μm; B) and E), the magnification was 200×. Scale bar = 50 μm; C) and F), the magnification was 400×. Scale bar = 25 μm. The blue staining was DAPI. G-J), Immunohistochemical staining of endarterectomized tissue. The neointimal layer of distal vascular wall tissues was assessed by immunohistochemical staining. G), Hematoxylin and Eosin (HE) staining; H), Single staining for α-SMA; I), Single staining for CD31; J), Double staining for CD31 and α-SMA; the magnification was 200×. Scale bar = 50 μm. K, L, M), Immunofluorescence staining of ELCs for the autophagic marker, LC3 (K), mitochondrial marker mitotracker red (L), and SOD-2 (M). K), The formation of autophagosomes (green punctate structures) was not detected. L), The normal filamentous mitochondrial reticulum (red punctate structures) was not detected. M), SOD-2 expression (green punctate structures) was not detected. The blue staining was DAPI. The magnification was 400×. Scale bar = 25 μm. ELCs = endothelial-like cells.

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