Increased expression of HIF-1α in HIV-Tg rats compared to wild type controls. A) Real-Time RT-PCR analysis of total mRNA and B) western blot analysis of total protein, extracted from lungs of HIV-Tg rats and age matched wild-type SD rats. Histogram below western blot image represents the average densitometric ratio of 135 kDa, HIF-1α to β-actin in wild-type and HIV-Tg rats. Statistical significance was calculated using a two-tail, independent, t-test. (* p < = 0.05) C) Immuno-histochemistry of paraffin embedded lung sections with anti-HIF-1α. Representative photomicrographs of immunostaining from wild-type and HIV-Tg group are shown. Original magnification: 60×.