Figure 1
From: Analysing the eosinophil cationic protein - a clue to the function of the eosinophil granulocyte
Identification of eosinophil granulocytes in peripheral blood by immunohistochemical detection of ECP. (A) Negative control (omission of primary antibody). Shown are peripheral leukocytes after fixation, incubation with alkaline phosphatase-anti-alkaline phosphatase (APAAP) with fast red substrate and counterstaining with Mayer's hematoxylin. The characteristic red immune-labelling reaction is absent. (B) Leukocytes are treated as in (A) but with addition of anti-ECP antibody. Peripheral leukocytes are visible but only the eosinophils have been stained for ECP. Original Magnification (X420).