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Figure 1 | Respiratory Research

Figure 1

From: Analysing the eosinophil cationic protein - a clue to the function of the eosinophil granulocyte

Figure 1

Identification of eosinophil granulocytes in peripheral blood by immunohistochemical detection of ECP. (A) Negative control (omission of primary antibody). Shown are peripheral leukocytes after fixation, incubation with alkaline phosphatase-anti-alkaline phosphatase (APAAP) with fast red substrate and counterstaining with Mayer's hematoxylin. The characteristic red immune-labelling reaction is absent. (B) Leukocytes are treated as in (A) but with addition of anti-ECP antibody. Peripheral leukocytes are visible but only the eosinophils have been stained for ECP. Original Magnification (X420).

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