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Figure 2 | Respiratory Research

Figure 2

From: Lipocalin 2 is protective against E. coli pneumonia

Figure 2

MMP9 expression in the lungs of E. coli-infected mice. Top. Western blot analysis for lipocalin 2 (Lcn2) and MMP9 (both antibodies diluted 1:1000) of whole lung lysates from uninfected and E. coli-infected (4 × 107 CFU E. coli H9049) wild-type (+/+) and Lcn2 knock-out (-/-) mice. Immunostaining for β-actin (dilution 1:5000) was included to assure equal loading of the samples. Bottom. Immunohistochemical staining for the neutrophil granule protein MMP9 (dilution 1:2000) on formalin-fixed lung sections of wild-type (A-C) and Lcn2 knock-out (D-F) mice. Only a few positive cells were found in the lungs of uninfected mice (A and D) whereas a larger number of cells were stained in the lungs of E. coli infected mice (4 × 107 CFU E. coli H9049/mouse) (B and E). No staining was seen when using a non-specific rabbit Ig as negative control (C and F).

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