Figure 4

PCBG induces activation of ERK in 1HAEo ^- airway epithelial cells. A. 1HAEo^- cells were challenged with 100 ug/ml of PCBG for eight hours and IL-8 release assessed by ELISA in the culture supernatants. Cells were pretreated for 1 hour with the ERK inhibitor PD 98059 or vehicle solution as indicated prior to the addition of PCBG. Data were analyzed with one-way ANOVA and posttest Bonferroni comparison (**denotes p < 0.01; ***denotes p < 0.001). B. 1HAEo^- cells were incubated with 100 ug/ml of PCBG for the indicated times, and phospho-p44/p42 and total p44/p42 were detected by western blot in the total cell lysate. C. Densitometry analysis of phospho- p44/p42 to total-p44/p42 ratio. D. 1HAEo^- cells were pre-incubated for 1 hour with different concentrations of TEMPO prior to stimulation with 100 ug/ml of PCBG for 10 minutes, phospho-ERK p44/p42 was detected by Western blot in the total cell lysate. Actin was shown as loading control. E. Densitometry analysis of phospho- p44/p42/Actin ratio. The data shown is representative of at least two independent experiments.