Figure 2

Intracellular calcium mobilization after PCBG stimulation. A. Airway epithelial cells (1HAEo-cells) were loaded with Fura-2AM and incubated with either 100 ug/ml of PCBG or with PAR-2 Peptide control (100 μM) for the indicated times and transient intracellular calcium release monitored by video fluorescence imaging. B. In additional experiments, airway epithelial cells were incubated with 100 ug/ml of PCBG. For one hour prior to the addition of PCBG, the cells were preincubated with various calcium and calcineurin disrupting agents (EGTA, BAPTA, or TEMPO) at the concentration indicated. IL-8 secretion was measured by ELISA in the supernatant of the cells after eight hours of incubation. C. Finally, airway epithelial cells were incubated with 100 ug/ml of PCBG for eight hours in the presence of cyclosporine A at the indicated concentration and IL-8 secretion measured by ELISA. Data were analyzed with one-way ANOVA and posttest Bonferroni comparison (*denotes p < 0.05; **denotes p < 0.01). The data shown are representative of three independent experiments.