Figure 1
From: Legionella pneumophila induces human beta Defensin-3 in pulmonary cells
L. pneumophilamediates hBD-3 release. (A, D) SAE cells, (B, E) A549 cells and (C, F) alveolar macrophages (aMϕ) were infected with L. pneumophila 130b (L. p.) for indicated time points (A-C) or for 24 h (D-F) as well as (E) incubated with TNFα [100 ng/ml] for 24 h. RT-PCR with specific hBD-3 primer was performed (A-C) and hBD-3 release was measured by ELISA (D-F). Representative gels out of three independent experiments are shown. ELISA results are means ± SEM of three independent experiments. *, p < 0.05 compared with uninfected control cells.