Figure 5
An H 4 R antagonist reduces mucus content and free collagen in the airways of allergen challenged lungs. Lungs from vehicle, JNJ 7777120 (20 mg/kg) and anti-IL-13 treated animals were sectioned and stained with alcian blue/PAS. (A) Lung histology with Alcian blue/PAS stain. 400× magnification (200× inset) n = 4. (B) Area of mucin staining per length of airway epithelium was calculated using image analysis software. (C) H4R antagonism inhibits collagen deposition in allergen challenged lungs. Lungs from vehicle, JNJ 7777120 (20 mg/kg) and anti-IL-13 treated animals were lavaged and resulting BALF was analyzed for free collagen levels. n = 3-8. Significance of each treatment group compared to control vehicle-treated animals is as follows: ** P < 0.01.