Figure 4
An H 4 R antagonist inhibits T cell chemokines and T cell influx in to allergen challenged lungs. Lungs from vehicle, JNJ 7777120 (20 mg/kg) and anti-IL-13 treated animals were sectioned and stained with anti-CD3+ antibody to highlight T cells. (A) Lung histology with CD3+ stain (400× magnification). n = 4. (B) CD3 + cells were quantified by a blinded observer. (C-E) Lung homogenates from the same animals were assayed for chemokine content and corrected for total protein. n = 8-10. Significance of each treatment group compared to control vehicle-treated animals is as follows: * P < 0.05, ** P < 0.01, *** P < 0.001