Figure 7

TGF-β1-induced CTGF promoter and SBE-SEAP reporter activities are not modulated by the BMPs. (A) The CTGF promoter pCT-sb was transiently transfected into NHLF, cells were then treated with 5 ng/ml TGF-β1 or 100 ng/ml BMP-4 or BMP-7 or with TGF-β1 in the presence of BMP-4 or BMP-7 in FGM containing 0.2% FBS. All assays were performed with 150000 cells/well in 2 ml total volume in 6-well plates and luciferase activity was measured after 24 h induction in 50 μl cell pellet. (B) MFB-F11 cells stably transfected with SBE-SEAP were stimulated with 5 ng/ml TGF-β1 or 100 ng/ml BMP-4 or BMP-7 or with TGF-β1 in the presence of BMP-4 or BMP-7 in serum-free DMEM. All assays were performed with 40000 cells/well in 100 μl total volume in 96-well plates and SEAP activity was measured after 24 h induction in 10 μl supernatant. Data are mean ± SD of five independent experiments. *, p < 0.05, as compared with unstimulated cells.