Figure 6

Nasal respiratory epithelia following LPC instillation. Examples of epithelial disturbance after LPC administration at the 60 min time point. Left side of tissue section is untreated and right side is treated. Goblet cells are those cells containing the compact darkly-stained contents (examples shown at dark arrows, untreated side). Scale bar = 50 μm. Low level effects, ranked at Level 1 (see Methods), displayed some loss of goblet cell mucins when compared to the untreated side (e.g. left panel). Disruption of the cell membrane junctions between cells, loss of some cilia and reduction in goblet mucin was ranked as Level 3 (e.g. middle panel, open arrow, treated side). An example of a region of complete exfoliation of epithelial layer produced by 1% LPC instillation is shown for comparison (Level 4, right panel).