LV gene transfer in nasal respiratory epithelium. (a) Histological examples of LV transduced (blue staining) cell types after 1 week. Left panel shows transduced ciliated and basal cells (solid arrow) and unciliated cells (open arrow, right panel). Safranin O staining, scale bar = 10 μm. LV Gene transfer at 1 week following 0.1% (b) and 1% (c) LPC variant pre-treatment. *p < 0.05 ANOVA against PBS, **p < 0.05 t-test C17:0 vs standard LPC (C16:C18), (n = 6/group). The proportion of respiratory cells types transduced following 0.1% (d) and 1% (e) LPC variant pre-treatment. Note: PBS is the same group of control animals in b-e) for Figure 1; a, b) for Figures 3, 5 and 7; and a-d) for Figure 4.